Table 1 Primers for rs3752462 genotyping
Primers (5′ - 3′) | Genotype pattern (bp) | Melting Temperature °C |
|---|---|---|
Forward Inner Primer | 287 (outer) 199 (T-allele) 140 (C-allele) | 69 |
476 AGGTGTGAGGTCAAAGCAAGCCTTGT 501 | ||
Reverse Inner Primer | ||
526 CGACCTCATTGAGAAGCCAGTGATTG 501 | ||
Forward Outer Primer | ||
387 CACCAAAAGGAAGGGGAGTTAAGACACC 414 | ||
Reverse Outer Primer | ||
673 GTTTGAGCAGCTGTGCATCAATTACACC 646 |