Table 1 Primers for the reverse transcription polymerase chain reaction (RT-PCR)
|
Gene (mouse or rat) | Primer sequence (5′ to 3′) |
|---|---|
| AT1 (mouse) |
F: GGAAACAGCTTGGTGGTGAT R: GGCCGAAGCGATCTTACATA |
| ACE1 (mouse) |
F: CAGTGTCTACCCCCAAGCAT R: TTCCATCAAAGACCCTCCAG |
| Nox1 (mouse) |
F: AGCCATTGGATCACAACCTC R: TGGATGGGATTTAGCCAAGA |
| Nox2 (mouse) |
F: TGTCATTCTGGTGTGGTTGG R: GAACCCCTGAGGAAGGAGAG |
| Nox4 (mouse) |
F: CTGGAAGAACCCAAGTTCCA R: ACTGGCCAGGTCTTGCTTTA |
| p22phox (mouse) |
F: AAAGAGGAAAAAGGGCTCCA R: CTGCCAGCAGGTAGATCACA |
| p47phox (mouse) |
F: ATCCCAACTACGCAGGTGAA R: TATCTCCTCCCCAGCCTTCT |
| GATA6 (mouse) |
F: GAGGACCTGTCGGAGAGCCG R: GCAAGTGGTCGAGGCACCCC |
| E2F3 (mouse) |
F: ATCCAAAGCTGTACCCTGGA R: TGGGTACTTGCCAAATGGAT |
| iNOS (mouse) |
F: CTCACTGGGACAGCACAGAA R: TGGTCAAACTCTTGGGGTTC |
| TNF-α (mouse) |
F: AGCCCCCAGTCTGTATCCTT R: CTCCCTTTGCAGAACTCAGG |
| IL-1β (mouse) |
F: GCCCATCCTCTGTGACTCAT R: AGGCCACAGGTATTTTGTCG |
| MCP-1 (mouse) |
F: AGGTCCCTGTCATGCTTCTG R: TCTGGACCCATTCCTTCTTG |
| IL-6 (mouse) |
F: AGTTGCCTTCTTGGGACTGA R: TCCACGATTTCCCAGAGAAC |
| Cox-2 (mouse) |
F: ATCCTGAGTGGGGTGATGAG R: GGCAATGCGGTTCTGATACT |
| VCAM-1 (mouse) |
F: TCATCCCCACCATTGAAGAT R: TGAGCAGGTCAGGTTCACAG |
| ICAM-1 (mouse) |
F: CCTGTTTCCTGCCTCTGAAG R: TTAAGGTCCTCTGCGTCTCC |
| GAPDH (mouse) |
F: GCATGGCCTTCCGTGTTCCT R: CCCTGTTGCTGTAGCCGTATTCAT |